| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Colorado Rapids Colorado Rapids Central 2007G Burgundy II |
CS23-23CG16-0025 | Massimo Baron |
| A2 | Colorado Rapids Colorado Rapids North 07G Burgundy I |
CS23-23CG16-0226 | James Roedel |
| A3 | Pride SC Pride SC United '07G |
CS51-51CG16-0041 | Brian Bronowski |
| A4 | Real Colorado RC '07G Express |
CS53-53CG16-0010 | Thomas Dueber |
| A5 | Colorado EDGE Soccer Club Colorado EDGE Legends 07G |
CS54-54CG16-0022 | Andy Bjorlow |
| A6 | Colorado Mountain United- Sanchez | CS22-22CG16-0001 | n/a |
| A7 | Boulder County United Boulder County United 07 Girls Academy Gold |
CS06-06CG16-0032 | Lindsay Hollingsworth |
| A8 | Broomfield Soccer Club Broomfield Blast SC - 2007 Girls Extreme |
CS08-08CG16-0025 | Tanner Sanders |
| A9 | Chivas Denver Soccer Academy Chivas Denver Soccer Academy - CS14 - GU16 |
CS14-14CG16-0001 | David Serrano Lopez |
| A10 | Colorado Rapids Colorado Rapids South 2007G Burgundy |
CS23-23CG16-0166 | Richard Mock |
| A11 | Colorado Rush Colorado Rush - 2007G Cinza |
CS24-24CG16-0009 | Brian De Herrera-Schnering |
| A12 | Colorado United Colorado United - 2007G Red |
CS25-25CG16-0030 | McKinzie Rees |