| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Colorado Rapids Colorado Rapids South 2007G Select |
CS23-23CG16-0165 | Ashleigh Fitteree |
| A2 | Colorado Rush Colorado Rush - 2007G Nero |
CS24-24CG16-0011 | Justin Trujillo |
| A3 | Colorado Rapids Colorado Rapids FC 06G Select - GU17 |
CS23-23CG17-0278 | Kevin Ireland |
| A4 | Colorado Rapids Colorado Rapids North 07G Select I |
CS23-23CG16-0224 | Kon Apostolopoulos |
| A5 | Colorado United Colorado United - 2006G White |
CS25-25CG17-0032 | John Senkosky |
| A6 | Arsenal Colorado Arsenal Colorado 2006G Academy |
CS01-01CG17-0041 | Graeme Oliver |
| A7 | Colorado Rapids Colorado Rapids South 2006G Select |
CS23-23CG17-0167 | Ashley Cabral |
| A8 | Arsenal Colorado Arsenal Colorado 2008G Academy |
CS01-01CG15-0034 | David Shaffer |
| A9 | Arsenal Colorado Arsenal Colorado 2007G Academy |
CS01-01CG16-0038 | David Shaffer |
| A10 | CO Elevation FC Co Elevation FC 07G Summit |
CS17-17CG16-0003 | Masoud Ashouri |